From: pjordan@cs.ualberta.ca (Peter Jordan)
Newsgroups: alt.drugs
Subject: Claviceps paspali references are HERE!
Date: 29 Aug 1994 23:57:22 GMT
Message-ID: <33tsl2$k1r@scapa.cs.ualberta.ca>

Interesting Chemical Abstracts References:
	CA: 71:P69351y	: describes mutants of strain NRRL 3027
	producing 2270 mg/l of ergoline compounds of which 85% were
	amides of lysergic and isolysergic acids. (Swiss patent application)

	CA: 77:P156333n : describes a method used to get 9.75g pure 
	lysergamides from a 10 litre fermentation originally containing
	an estimated 15g.

	CA: 94:13992r : reference Indian Drugs; 1980; 17(8) 228-31 Eng.
	CA: 90:118108c : reference Indian Drugs; 1979; 16(4) 88-93 Eng.
	Very interesting>:-||

	CA: 102:219574y : Serbo-Croat areticle describing 2.2g/l prod.
	by C. paspali Stevens and Hall (1984).

Nature reference:	Nature: 187:238-239. I can't remember this one 
but I have written down "-production by C. paspali in submerged culture" .

Lloydia references: lots; just go check the indexes for each volume.

An absolute Gem from 1916:	Life History and Poisonous Properties
	of Claviceps paspali; H. B. Brown (Mississippi Agricultural 
	Experiment Station); Journal of Agricultural Research ,vol. 7(9), 
	pgs 401-405. describes on brief glance through it --- germination
	of the yellowish-grey sclerotia found on Paspalum dilatatum Poir.
	observed in the region of the Mississippi Agricultural College.
	Also contains reference to Stevens and Halls' original article (1910). 


	"So there you have it gentlemen, what more evidence do we need"

	Yow!!! If you find this usefull/helpfull/interesting, let
me know and I'll post a whole lot more. My willingness to post this stuff
is directly correlated to the magnitude of the responses I get.

Thanks very much for the encouragement from those who responded to the
earlier postings. (Two from Germany and Two from USA).

P.S. If you find a good culture of this stuff, I would LOVE to have some.

P.P.S. If you take your rights, you'll never have them.

		ME!
		Peter Jordan

=============================================================================

From: pjordan@cs.ualberta.ca (Peter Jordan)
Newsgroups: alt.drugs
Subject: More Claviceps references!
Date: 31 Aug 1994 20:16:23 GMT
Message-ID: <342oen$o4n@scapa.cs.ualberta.ca>

Hi again, Enjoy these goodies:

	* Biotechnological Exploitation of the Ergot Fungus
 (Claviceps Purpurea); Karl Esser and Andrea Duvell; Process
 Biochemistry, August 1984 pgs 142-149.
Synopsis: "The alkaloids of the ergot fungus C. purpurea and related 
species already known as drugs in the middle ages are still finding 
many uses in medical therapy (he he). Since the supply of natural grown 
ergots is not sufficient, the biotechnological production of ergot
alkaloids has gained in importance. This requires not only an undestanding
of physiological and environmental conditions, but also concerted breeding
in order to increase and stabilize the production level."

	* Biology of Claviceps; Willard A. Taber; Chapter 15 (sorry -
i don't remember which book this came from, but it should be indexed 
under Taber in Biological Abstracts) pgs 449-486.
"If one desires isolates of C. paspali (which are high producers of
simple amides), one must go to paspalum grass. ... C. paspali 
differs from all other species in possesing a yellowish tan cauliflower-
shaped sclerotium rather than the purplish banana-shaped sclerotium, and
it has been suggested that this species be trasnsferred to the genus 
Mothesia. "

	*The Ergot Alkaloids; A. Stoll and A. Hofmann (THE); 
Chapter 21, The Alkaloids, Manske (ed. ?) vol. VIII, pgs 725-779+.
Describes lots and lots and lots of chemical detail regarding 
everything from biogenesis to complete chemical synthesis as a means 
of confirming structure. Also has a section completely devoted to 
"Derivatives of Ergot Alkaloids" in which the following processes 
for synthesizing amides are discussed:
		=> The azide process.
		=> DMF-SO3 mixed anhydride method.
		=> mixed Lysergic acid trifluoroacetic anhydride.
		=> Lysergic acid chloride hydrochloride method.
		=> N,N'- carbonyldiimidazole as condensing agent (Best IMHO).


My fingers are tired, PLEASE do not rip any articles or pages 
from journals/books, as this is the most insidious kind of censorship
(something a pig might do).

		Untill Later;
		Peter Jordan

P.S. The P.P.S. of my previous posting should have read:
	"If you DON'T ........"