|
von Hungen K, Roberts S, HILL DF.
“Interactions Between Lysergic Acid Diethylamide And Dopamine-sensitives Adenylate Cyclase Systems In Rat Brain”.
Brain Res. 1975;94:57-66.
|
|
Abstract
Investigations were carried out on the interactions of the hallucinogenic drug, d-lysergic acid diethylamide (d-LSD), and other serotonergic antagonists with catecholamine-sensitive adenylate cyclase systems in cell-free preparations from different regions of rat brain. In equimolar concentration, d-LSD, 2-bromo-d-lysergic acid diethylamide (BOL), or methysergide (UML) strongly blocked maximal stimulation of adenylate cyclase activity by either norepinephrine or dopamine in particulate preparations from cerebral cortices of young adult rats. D-LSD also eliminated the stimulation of adenylate cyclase activity by equimolar concentrations of norepineprine or dopamine in particulate proparations from rat hippocampus. The effects of this hallucinogenic agent on adenylate cyclase activity were most striking in particulate preparations from corpus striatum. Thus, in 10 mcM concentration, d-LSD not only completely eradicated the response to 10 mcM dopaminein these preparations but also consistently stimulated adenylate cyclase activity. L-LSD (80 mcM) was without effect. Significant activation of striatal adenylate cyclase was produced by 0.1 mcM d-LSD. Activation of striatal adenylate cyclase by either d-LSD or dopamine was strongly blocked by the dopamine-blocking agents trifluoperazine, thioridazine, chlorpromazine, and haloperidol. The stimulatory effects of d-LSD and dopamine were also inhibited by the serotonin-blocking agents, BOL, 1-methyl-d-lysergic acid diethylamide (MLD) and cyproheptadine, but not by the beta-adrenergic-blocking agent, propanolol. However, these serotonin antagonists by themselves were incapable of stimulating adenylate cyclase activity in the striatal preparations. Several other hallucinogens, which were structurally related to serotonin, were also inactive in theis regard, e.g., mescaline, N,N-dimethyltryptamine, psilocin, and bufotenine... (ABSTRACT TRUNCATED HERE)
|
| # |
| Submit Comment |
|
