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Kubinski H, Andersen PR, Kasper CB.
“Effects of Neuropharmacological Agents on Microsomal Membrane Influence on Deoxyribonucleic acid-Membrane Interactions In Vitro.”.
Neuropharmacology. 1974;l3(10-11):969-975.
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Abstract
The effects of several neuroactive compounds on microsomal membrane - DNA interactions were studied in-vitro. Methods Rat liver microsomal membrane was isolated and the effects of chlorpromazine (SK+F), haloperidol (Haldol, McNeil), desipramine (Geigy), dopamine, L-DOPA, diethezine (SK+F), barbital morphine, LSD, (Sigma), mescaline (Sigma), orphenadrine (Riker), phenindamine, Tensilon (edrophonium) neostigmine (Sigma), decamethonium, (+)-tubocurarine, succinylcholine (Aucctine), tetracaine (Winthrop), dimethisoqllill (SKiF) iovocaine, cyclo-methycaine, holocaine (Winthrop) and dibucaine (all 400 mcg/ml) on membrane density and its ability to accomodate DNA (E.coli) binding were determined by previously described methods. Results All drugs decreased microsomal membrane density (espec-ially chlorpromazine, tetracaine and tubocurarine) except for decamethomine bromide which increased it and haloperidol, barbital, LSD, mescaline, neostigmine, Anectine and novocaine which had no effects. E.coIi, DNA binding to microsomal mem-branes was increased by all drugs (especially dibucEine, cyclo-methycaine and dimethisoquin) except for chlorpromazine, desi-pramine, L-DOPA, diethezine, morphine, and neostigmine which decreased binding and barbital, LSD, mescaline, orphenadrine, Tensilon, decamethonium and tubocurarine whicl; had no effect In 3/5 cases studied the decrease in membrane density was corr-elated with reduced membrane protein content
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