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Szepesy L, Feher I, Szepesi G, Gazdag M.
“High-Performance Liquid Chromatography of Ergot Alkaloids”.
J.Chromatog.. 1978;149:271-80.
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Abstract
The analysis of Ergot alkaloids by HPLC is reported. Ergotamine, ergocristine and dibydroergotamine were obtained by plant extraction. Ergocornine, ergocryptine and ergometrine were isolated from a fermentation process. The dihydro derivatives listed below were obtained from both sources. The extracts were applied to LiChromosorb or Silica SI or LiChrosorb or Silica RP-8 columns with CHCl3-EtOH (95.5), CHCl3-n-hexane-EtOH (40:40:10) and CHCl3-MeOH as eluants. The compounds listed below were isolated. The internal standards 1-phenyl-2,3-dimethyl-4-dimethylamino-5-pyrazolone- and 2 propylthiocarbamoyl-4-pyridine were for ergo/amine-containing samples and ergocristine-containing samples respectively. The individual ergotoxine alkaloids listed below were separated on reversed-phase columns using bonded-phase packings containing C2, C8 and C18 alkyl groups. A high-efficiency micro particulate reversed-phase column was used to separate the active compounds as stereo and structural isomers. Lysergic acid Ergometrine maleate Ergometrinine Ergotamine tartarate Ergocornine Ergocryptine Ergocristine Ergotaminine Ergocorninine Ergocryptinine Ergocristinine Dihydroergotamine tartarate Dihydroergocornine methanesulphonate Dihydroergocryptine methanesulphonate Dihydroergocristine methanesulphonate
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