Erowid References Database
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Hirsch JD.
“Selective inhibition of [3H]lysergic acid diethylamide binding to mouse brain membranes by ethanol”.
J. Pharm. Pharmacol.. 1981;33:475-477.
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Abstract
Recent reports have indicated that ethanol inhibits the binding of a number of ligands to brain membranes in vitro (Ross et al 1977; Ciofalo 1978, 1980; Fairhurst & Liston 1979; Hruska & Silbergeld 1980; Shanley ct al 1980). During screening of drugs dissolved in ethanol, I observed that 5-hydroxytryptamine (5-HT) receptors in mouse brain membranes labelled with [3H]lysergic acid diethylamide (LSD) and [3H]5-HT were also sensitive to this alcohol. Further work revealed that 5-HT receptors labelled with [3H]LSD were about three times more sensitive to ethanol than those labelled with [3H]5-HT. Moreover, ethanol lowered the Bans for [3H]LSD binding while increasing the affinity (Kd) for the ligand, suggesting uncompetitive inhibition. I observed no change in the Hill coefficient for [3H]LSD binding in the presence of ethanol. [3H]LSD binding to dopamine receptors in mouse brain membranes was also inhibited by ethanol. A preliminary report of this data has appeared (Hirsch 1980).
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