Erowid References Database
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Kleven MS, Dwoskin LP, Sparber SB.
“Lysergic Acid Diethylamide (LSD) attenuates 5-hydroxytryptophan (5-HTP) induced increases in 5-hydroxyindoleacetic acid (5-HIAA) in ventricular perfusate collected during operant behavior”.
Federation Proceedings. 1982;41(4):1047.
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Abstract
Cannulas were implanted in the lateral ventricles of rats responding on a fixed ratio 15 operant for food reinforcement. During an 80 min session, ventricles were perfused with saline (0.9%, 2.3 nM CaCl2; 15 mcl/min). Perfusate, collected for 20 min into acid buffer, was analyzed for dihydroypheylacetic acid (DOPAC), homovanillic acid (HVA), or 5-HIAA by loading directly onto a high performance liquid chromatograph: (ODS C18 reverse phase column; 25 degrees C); 90% 0.1 M KH2PO4 (pH3.0), 0.1 M Na2EDTA, 0.25 mM sodium octane sulfonate, and 10% MeOH; glassy carbon electrode (+ .80V vs Ag/AgCl). Administration of LSD (400 mcg/kg, ip) suppressed behavior without affecting DOPAC, HVA, or 5-HIAA in perfusate. A behaviorally inactive dose of 5-HTP (5 mg/kg, ip) resulted in an imediate increase in 5-HIAA without affecting DOPAC or HVA. The increase in 5-HIAA in this manner may be insensitive to transient or local changes; however, injections of 5-HTP amplifies the apperance of 5-HIAA so that drug effects can be demonstrated.
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