|
Cody JT, Schwarzhoff R.
“Fluorescence polarization immunoassay detection of amphetamine, methamphetamine, and illicit amphetamine analogues”.
J Anal Toxicol. 1993 Jan-Feb 08;17(1):23-33.
|
|
Abstract
The Abbott Diagnostics Amphetamine/Methamphetamine II and Amphetamine Class reagents were evaluated on the Abbott TDx for cross-reactivity to amphetamine and methamphetamine stereoisomers, several of their metabolites, and various illicit analogues, including 2-methoxyamphetamine, 4-hydroxymethamphetamine, 2,5-dimethoxy-amphetamine DMA, 4-bromo-2,5-dimethoxyamphetamine DOB, 4-bromo-2,5-dimethoxy-beta-phenethylamine BDMPEA, 3,4,5-trimethoxyamphetamine TMA, 3,4-methylenedioxy-amphetamine MDA N,N-dimethyl-3,4-methylenedioxy-amphetamine, N-hydroxy-3,4-methylenedioxyamphetamine N-OH MDA, 3,4-methylenedioxymethamphetamine MDMA, 3,4-methylenedioxyethylamphetamine MDEA, 2,5-dimethoxy-4-ethylamphetamine DOE, 2,5-dimethoxy-4-methylamphetamine DOM, and mescaline in concentrations ranging from 100 to 100,000 ng/mL. Results demonstrate the utility of this assay for detection of several of the above compounds unfortunately many are still not detectable. Significant differences were observed between the Amphetamine/Methamphetamine II and Amphetamine Class reagents, particularly regarding their cross-reactivity to over-the-counter medications. Detection of the drugs amphetamine, methamphetamine, and the illicit analogues is not enhanced with the Amphetamine Class reagents, and unless detection of the over-the-counter compounds is of interest, these reagents are a poor choice compared to the Amphetamine/Methamphetamine II reagents. Cross-reactivity of some of the illicit analogues is such that the assay can reliably be used for the routine screening of these compounds.
|
| # |
| Submit Comment |
|
