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Concheiro M, de Castro A, Quintela O, López-Rivadulla M, Cruz A.
“Determination of MDMA, MDA, MDEA and MBDB in oral fluid using high performance liquid chromatography with native fluorescence detection”.
Forensic Sci Int. 2005 Jun 09;150(2-3):221-6.
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Abstract
This paper describes the analytical methodology for the determination of MDMA, MDA, MDEA and MBDB in oral fluid. After a liquid-liquid extraction, the analysis was carried out by high performance liquid chromatography HPLC, with fluorescence detection. The detector wavelength was fixed at 285 nm for excitation and 320 nm for emission. The mobile phase, a mixture of phosphate buffer pH=5 and acetonitrile 75:25, and the column, Kromasil 100 C8 5 microm 250 mm x 4.6mm, allowed good separation of the compounds in an isocratic mode in only 10 min. The method was validated and showed good limits of detection 2 ng/mL and quantitation 10 ng/mL for all the amphetamine derivatives. No interfering substances were detected. A stability study of these compounds in oral fluid stored at three different temperatures -18, 4 and 20 degrees C over 10 weeks was conducted, showing a time-dependent degradation of the four compounds.
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