Erowid References Database
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Datta RK, Ghosh JJ.
“Mescaline-induced changes of brain-cortex ribosomes Mescaline demethylase activity of brain-cortex soluble supernatant”.
Naunyn Schmiedebergs Arch Pharmacol. 1977 Feb 25;296(3):297-300.
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Abstract
Brain-cortex slices demethylate mescaline and p-methoxyacetanilide, a reference O-demethylating substrate, though the rate of demethylation of mescaline is about one third that of the reference substrate. The demethylase activity is localized mostly in the soluble supernatant 105 000 x g. It is purified 47-fold with respect to the demethylation of mescaline by ammonium sulfate precipitation and DEAE cellulose chromatography. The partially purified demethylase, which is stable for 3-5 days at -5 degrees C in the presence of dithiothreitol and glutathione and is inhibited by p-chloromercuribenzoate, has maximal activity at pH between 7.2 and 8.0. It demethylates mescaline into 3,4-dimethoxy-5-hydroxyphenethylamine and 3,5-dimethoxy-4-hydroxyphenethylamine and some unidentified derivatives.
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