2002, by 3base
isolation of piperine (black
pepper, EtOH, KOH)
isolation of piperine from black
pepper (black pepper, EtOH, KOH, soxhlet)
isolation of piperine from black pepper (black
pepper, IPA, CaCO3)
isolation of piperine from black pepper (black
pepper, CH2Cl2)
isolation of natural piperine (pepper,
EtOH, KOH, soxhlet)
extraction and isolation (piper
nigrum, EtOH)
piperine and piperiline
extraction (black pepper, EtOH, soxhlet, hexane:ether column)
piperine form piper longum (piper
longum, CH2Cl2:MeOH, PE:EtOAc column)
oleoresin of pepper
piperine from
resin of black pepper (black pepper resin, EtOH, urea)
source of
piperine (black/long pepper/oleoresin, BuOH, hexane, alumina,
charcoal)
oleoresin of pepper [03] (black
pepper, ether)
further recipes and references
place 15g (1) of ... ground black pepper in a 250mL round-bottomed
flask,
add 150mL of 95% ethanol and 5 boiling chips (2), and heat at
reflux for 2h.
filter the mixture by suction filtration and then concentrate the
filtrate to a
volume of 10-15mL by simple distillation or by use of a rotary
evaporator.
to 10mL of a 10% solution of KOH in 95% ethanol contained ina 125mL
erlenmeyer flask add the concentrated pepper extract. heat the
resulting
solution and add water dropwise. a yellow precipitate forms. add
water
until no more solid appears to form and then allow the mixture to
stand at
least overnight (3). collect the solid by suction filtration and
recrystallize it
with 10-20mL of acetone (4).
(1) this extraction may be scaled up to twice the amounts
specified without
difficultiy. if a soxhlet extractor is available, this would be
an apparatus superior
to a standard reflux setup.
(2) boiling chips are necessary to prevent serious bumping.
(3) it is best to allow piperine to completely precipitate out by
allowing the
mixture to stand until the next laboratory period.
(4) in our hands ca. 0.6g of piperine, mp 127-128°C, was
collected upon
recrystallization.
[05, page 527-528]
procedure
10g black pepper is ground to a fine powder and extracted with
150mL 95%
ethanol in a soxhlet extractor for 2h. the solution is filtered
and concentrated in
vacuo on a water bath at 60°C. 10mL 10% alcoholic KOH solution
is added ...
and after a while decanted from the insoluble residue. the
alcoholic solution is
left overnight, whereupon 0.3g yellow needles are deposited, mp
125-126°C.
TLC of black pepper extract
the crude extract is spotted on a thin-layer plate (silica gel GF254)
and developed with benzene:EtOAc 2:1.
detection:
(1) UV365 shows blue fluorescence of piperine
(2) spraying with anisaldehyde-sulfric acid reagent, prepared by
mixing 0.5mL
anisaldehyde with 10mL glacial acetic acid, 85mL MeOH, and 5mL
concentrated
sulfric acid. this solution is sprayed on the plate, which is
then heated at 110°C
for 10min. piperine appears as a yellow spot, Rf=0.25.
[06, page 233-236]
piperine, a very weakly basic substance, can be isolated from
a variety of peppers
by extraction with alcohol. ... piperine, along with a small
amount of its Z,E isomer,
accounts for about 10% of the weight of black pepper.
procedure
place 15g of ground black pepper and 1g of powdered CaCO3 (1) in
a 250mL
boiling flask, add 100mL of IPA, and, after fitting the flask
with a reflux condenser,
boil the mixture for about 1h on the steam bath. at the end of
the heating period,
filter the mixture by gravity into a 125mL erlenmeyer flask,
clean the 250mL
boiling flask, and return the filtrate to the boiling flask. fit
the boiling flask with a
distillation adapter and condenser, and boil off all but about 10mL
of the IPA (2).
transfer the residual solution from the boiling flask to a 25mL
erlenmeyer flask,
and set the flask aside to cool for crystallization of piperine (3,4).
collect the product
by suction filtration, using small portions of MeOH to rinse the
flask and wash
the product. yield: about 0.5g.
(1) the addition of CaCO3 should prevent the extraction of acidic
components of pepper
(2) IPA boils at 80°C, only a little below the maximum
temperature attainable on
the steam bath. to make the distillation proceed quickly, clamp
the boiling flask so
that it is well down in the rings of the steam bath, and drape a
towel over the flask
and the steam bath to make a tent that will hold steam around the
top of the flask.
(3) crystallization occurs slowly, and the flask must be allowed
to stand for
at least 24h.
(4) alternatively, add 25mL of water to the IPA solution of
piperine, allow the mixture
to stand for at least 24h so that precipitation will be complete,
collect the solid by
sucction filtration, and recrystallize it from either IPA or
acetone.
[04, page 340-341]
add 5g of pure ground pepper and 10mL of CH2Cl2 to 50mL round-bottomed
flask.
use the round-rottomed flask as the basis for a reflux apparatus
having a water-cooled
condenser and heating mantle. heat the sample to reflux, and then
maintain a gentle
reflux for 20min. after the required reflux period, lower the
heating mantle and allow the
reflux apparatus to cool for 5min. suction filter the slurry with
the aid of a 4.5cm buchner
funnel, washing the pepper grounds once with 5mL of CH2Cl2.
remove 2 or 3 drops of
the extract and place it in a capped vial for use in the TLC
analysis.
trituration/isolation
transfer the extract obtained above to a clean 25mL round-bottomed
flask and
concentrate in vacuo. the resulting olive-brown, viscous oil
should be cooled in an
ice-bath and then 3mL of cold ether added to the oil while gently
stirring for 3-4min.
some piperine may percipitate at this point, but remove the
solvent in vacuo anyway.
once again coole the resulting oil in an ice-bath and then add 3mL
of cold ether to the
oil while gently stirring to promote the percipitation of
piperine. allow the flask to cool
for an additional 10min with occasional stirring. isolate the
straw-yellow crystals of
crude piperine by suction filtration with the aid of a 1.5cm
hirsch funnel. wash the
crystals twice with 2mL portions of cold ether. place a small
portion of the filtrate in a
capped vial for use in the TLC analysis.
recrystallization
place the crude piperine isolated above into a 13x100mm test tube
and dissolve it in a
minimum amount of hot 3:2 acetone:hexane solution. once all the
solid has dissolved,
allow the test tube to sit undisturbed for 15min at room
temperature. rod-like, yellow
crystals of piperine should be present. cool the solution for an
additional 30min in an
ice-bath before isolating the purified piperine by suction
filtartion with the help of a 1.5cm
hirsch funnel. wash the crystals once with a 2mL portion of cold
ether, allow them to air
dry for several minutes. typically, yields of approximately 2% or
100mg are obtained.
the melting point of the purified piperine now can be determined
and the identity of the
product confirmed by mixed melting point, TLC analysis, or
spectral analysis.
TLC analysis
transfer a small portion of the purified crystals to a small vial
and dissolve them in a
drop or two of acetone. prepare a silica-gel TLC plate for the
spotting of 4 samples
(kieselgel 60 F254 plates having a 0.2mm coating thickness are
recomended).
with the aid of a capillary, place a sample of the crude oil
remaining from the extraction
procedure, a sample of the filtrate from the trituration/isolation
procedure, the sample
prepared from the purified piperine, and a sample of a piperine
standard on separate
points of the thin-layer plate. develop the plate using 3:2
acetone:hexane.
visualize under UV illumination and stain in an iodine chamber.
[01]
grind 25g fresh peppercorns to a fine powder, place in a
soxhlet thimble, and extract
with 100mL ethanol for 90 minutes. cool the resulting solution,
filter if necessary, and
concentrate on the rotary evaporator. keep the water bath below
60°C during the
concentration. dissolve the residue in 25mL 10% alcoholic KOH.
decant the solution
if any residue remains. cool the solution in an ice bath, and add
water dropwise
(about 30mL will be required) to precipitate the piperine.
collect the piperine on a
sintered glass funnel, and dry it on the vacuum pump.
... recrystallization from acetone:hexane 3:2 will produce
cleaner material.
[14]
10kg dried fruits of p nigrum l ... were crushed and
extracted 5x with EtOH at room
temp. after removal of solvent in vacuo, the syrupy residue was
left overnight at room
temp. when a white crystallizate sept out, which was filtered. on
recrystallization from
MeOH it formed 50.54g fine needles, mp 128-129°, and was
identified as piperine.
[19]
750g dry fruits of black pepper were ground and extracted with
2.5L ethanol, in a
soxhlet apparatus for 72h. the extract was concentrated under
reduced pressure
in a rotary evaporator to leave 8g of a dark brown oil. this
material was fractionated
on a silica gel column, eluting with hexane + diethyl ether (1 +
2 by volume).
the fractions obtained were combined according to their
similarities as analysed by
thin layer chromatography (TLC) and this led to the isolation of
2g piperine and 0.3g
piperiline. these compounds were recrystallised with a mixture of
dichloromethane
and hexane and the physical and spectroscopic data obtained were
in accordance
with those reported in the literature.
[09]
p longum ... 500g dried fruits were powdered and
extracted 120h with CH2Cl2:MeOH
1:1. the concentrated extract (1.45g) was chromatographed over
silica gel, the column
being eluted with pet. ether and pet. ether-EtOAc mixture. the
following compounds were
eluted according to the increasing order of polarity: 120mg
tridecyldihydro-p-coumarate,
126mg eicosanyl-(E)-p-coumarate, 132mg pellitorine, 67mg
piperlongumine and 148mg
piperine.
[35]
... black pepper ... ground ... berries must be extracted
repeatedly(percolated) with
volatile solvents: alcohol, acetone or ether. concentration of
the solutions and removal
of the solvent in vacuo yields the socalled oleoresin of pepper.
quantitative composition of the oleoresin depends upon the
solvent used. oleoresin
of pepper generally contains these compounds: 1. piperine,
... 2. chavicine, ... an
isomer of piperine ... 3. other piperidides, ... 4.
the volatile oil, ... 5. a volatile alkaloid,
... present in samll quantities only. 6. resins, not
identified.
[08, page 144]
a reaction vessel was charged with 0.9kg urea, 3.7kg ethanol (or
2.7kg methanol)
and 0.3kg of the resin of black pepper to form a mixture. the
mixture was refluxed
for approximately 1h. the refluxed mixture was cooled overnight
to 0-5°C. the cooled
mixture was then filtered to remove solids (e.g wax, urea, fatty
acids). the filtrate was
then concentrated to obtain 180g of piperine and some unreacted
urea. the 180g of
piperine was recrystallized by adding ethanol. the piperine
crystals were collected
by filtration and dried to yield approximately 75g of 98% pure
piperine.
[12]
black pepper oleoresin or long pepper oleoresin is used as the
source of piperine.
ground up black pepper or long pepper can also be used
to 35L buthanol/hexane mixture, 35kg black pepper oleoresin is
added and
heated to 40°C. the mixture is then cooled and filtered. the
percipitate is washed
with buthanol/hexane mixture to obtain crude piperine. the crude
piperine is
dissolved in ethanol at 60°C and treated with alumina and
charcoal by stirring.
it is then filtered and concentrated under vacuum to obtain a
pale yellow crystalline
powder, melting range: 128-131°C, min.98% pure piperine (by HPLC).
[13]
with EtOH [26, (1)]
with MeOH [27]
with EtOAc: recipe 1,
recipe 2
with CHCl2: recipe
1 [10], recipe 2
with acetone: [hive post 282850 on 2002-03-15 by Antoncho]
microwave-assisted extraction of piperine from piper nigrum [15]
extraction of piperine from piper nigrum (black pepper)
by hydrotropic solubilization [31]
process for extraction of piperine from piper species [07]
extraction and description of the piperines in pepper [16]
isolation of piperine from black pepper
[http://www.wiu.edu/users/mftkv/Chem332/Experiment3.html]
... extracted ground black pepper with acetone ...
agricult food chem, 1981 29, 115; su, horvat
(mentioned in [21])
... piper nigrum fruit extract and its main alkaloid, piperine [17]
... pepper raw spice, oleoresins, and soluble seasonings.
piperine is extracted into ethylene dichloride ... [11]
... pepper or piper longum ... is extracted ... [34]
process for preparing piperine [02]
immersing extraction of pepper [32]
CO2 extraction, up to 9.2% piperine from black pepper(madagascar)
[23]
liquid CO2 extraction of piperine ... of pepper [33]
[hive]
http://www.the-hive.ws/
[pubmed]
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
[01]
journal of chemical education 1993 70(7): 598-599
"isolation of piperine from black pepper"
william w epstein, david f netz, jimy l seidel
[02]
patent CN1294127, 2001-05-09 (chinese)
"process for preparing piperine"
xiaoming lin, zaifeng shi, zhenfan sun
(Lutianyuan Hi-Tech Development Co., Ltd., Hainan, Peop. Rep.
China)
Faming Zhuanli Shenqing Gongkai Shuomingshu
abstract (from esp@cenet): a process for
preparing piperine includes
immersing extraction of pepper is mixed organic solvent,
distilling to
separate out organic solvent to obtain liquid mixture of pepper
oil resin
and piperine, cooling to separate out the pepper oil resin, and
further
purifying. Its advantages are simple process, high extraction
rate and
separation effect, and high purity.
abstract (from CAS): the process
comprises extg. pepper with mixed
solvent at 25-40° for 1-6 h, filtering, concg., crystg., and
recrystg. with
dichloroethylene or Et acetate. the mixed solvent is ethanol,
methanol,
acetone, Et ether, Et acetate, dichloromethane, benzene, and/or
chloroform.
keywords (from CAS): piperine isolation
pepper
[03]
king's american dispensatory 1898
m d harvey wickes felter, phr m ph d john uri lloyd
[04]
"techniques and experiments for organic chemistry"
6.edition 1998
addison ault (cornell college); universityscience books,
susalito, california
ISBN 0-935702-76-8
[05]
"laboratory experiments in organic chemistry"
3.edition 1979
jerry r. mohrig, douglas c. neckers
d. van nostrand company, new york
ISBN 0-442-25471-7
[06]
"natural products - a laboratory guide"
2.edition 1991
raphael ikan; academic press, inc; ISBN 0-12-370551-7
[07]
patent US6365601, 2002-04-02
"process for extraction of piperine from piper
species"
RAMAN GIRIJA (IN); GAIKAR VILAS GAJANAN (IN)
COUNCIL SCIENT IND RES (IN)
abstract: the present invention relates
to a process for extraction
of piperine of formula I from the fruits of piper species,
comprising
the steps of:contacting the fruit of piper species with aqueous
hydrotrope solution at a temperature in the range of 0-100 C. and
separating the solution from the solid residue by known methods,
and
recovering piperine from the solution by known methods.
[08]
"the essential oils" volume five 1952,
ernest guenther
d van nostrand company, toronto - new york - london
[09]
pest
management science 2000 56(2): 168-174
"synthesis and insecticidal activity of new amide
derivatives of piperine"
vanderlúcia fde paula, luiz c de a barbosa, antônio j demuner,
dorila piló-veloso, marcelo c picanço
abstract: the natural
lipophilic amides piperine and piperiline were isolated
from piper nigrum l (piperaceae). piperine was hydrolysed into
piperic acid
(85% yield) which was converted into 16 amides (28-89% yield).
the contact
toxicity of all synthetic amides, and also that of piperine and
piperiline, at the
dose 10 µg per insect, was evaluated for the brazilian
economically important
insects ascia monuste orseis latr, acanthoscelides obtectus say,
brevicoryne
brassicae l, protopolybia exigua de saus and cornitermes cumulans
kollar.
the results demontrated that the insects have different
sensivities to the various
amides, with mortality ranging from 0 to 97.5% according to the
compound
and insect species.
[10]
phbcan99.rtf from http://www.uni-bayreuth.de/departments/oc1/teaching/
"isolierung von piperin aus schwarzem pfeffer und
abbau zur piperinsaeure"
ANALYTISCHES PRAKTIKUM fuer Biochemiker (Stand: September 1999),
page 16-18
[11]
j assoc off anal chem 1987 70(1): 112-3
"UV spectrophotometric determination of piperine in
pepper preparations:
collaborative study."
t lupina, h cripps
abstract: eight
collaborating laboratories performed replicate analyses for
piperine
on 5 samples representing pepper raw spice, oleoresins, and
soluble seasonings.
piperine is extracted into ethylene dichloride and measured at
maximal
absorbance 342-345 nm with a UV light source. piperine content is
calculated
using an absorbance factor derived from piperine. Intralaboratory
coefficients
of variation (CVo) ranged from 0.5 to 3.1%; interlaboratory
coefficients of
variation (CVx) ranged from 3.0 to 5.8%. the method has been
adopted as an
official method of the american spice trade association and as an
official first
action method by AOAC.
[pubmed, PMID: 3558260]
[12]
patent US6054585, 2000-04-25
"process for making high purity piperine for
nutritional use"
muhammed majeed, vladimir badmaev; sabinsa corp
abstract: the present invention
relates to a process of making high purity
piperine for nutritional and nutraceutical application. this
process recovers
piperine from piperine-containing oleoresin by using isourea,
urea or a
urea derivative to remove organic matter other than piperine from
the
oleoresin. preferrably, the process recovers piperine from
oleoresin of fruit
of piper nigrum or piper longum. more preferably, the process
recovers
piperine from oleoresin of fruit of piper nigrum, i.e. black
pepper.
[13]
patents US5744161, US6054585, US5972382, US5536506
[14]
http://www.chemistry.uvic.ca/chem465-66/465e03piperine.pdf
from
http://www.chemistry.uvic.ca/chem465-66/465organic.htm
[15]
ind eng chem res 2002 41(10): 2521-2528
"microwave-assisted extraction of piperine from
piper nigrum"
girija raman, vilas g gaikar
abstract: a novel
microwave-assisted technique (MAE) for the extraction of
piperine from coarsely powdered black pepper (Piper nigrum) was
studied.
the parameters such as nature of the solvent, microwave energy
input, and
solid loading were optimized. the mechanism of the enhancement of
extraction
rates was investigated by microscopic studies of the irradiated
material.
studies have revealed that dielectric heating of the polar
cellular matrix resulted
in remarkable swelling and coalescence of the oil cells and other
constituents.
the resulting pressure, built-up within the cell, breaks open the
cell, releasing
the constituents and providing easy access for solvent
penetration and
subsequent solubilization of piperine and other substances.
because the cell wall
is mainly composed of slightly ionic cellulose, there is a marked
increase in the
dielectric heating rates. rapid degradation of the cellulosic
cell wall occurs,
thereby further increasing the permeability of the wall toward
solvent penetration.
selective extraction of piperine in nonpolar solvents to the
extent of 94% with a
purity of 85% has been achieved. the MAE procedure is simple,
rapid, and reliable.
[16]
bull soc chim 1877 27(2): 290
"the extraction and description of the piperines in
pepper"
p caseneuve, o caillol
[17]
planta medica 1999 65: 600-3
"stimulation of mouse melanocyte proliferation by
piper nigrum fruit extract and its main alkaloid, piperine"
zhixiu lin, j r s hoult, dorothy c bennett, amala raman
abstract: during a herbal screening
programme to find potential repigmenting agents
for the treatment of vitiligo, piper nigrum l. fruit (black
pepper) extract was found to
possess growth-stimulatory activity towards cultured melanocytes.
its aqueous extract
at 0.1mg/ml was observed to cause nearly 300 % stimulation of the
growth of a cultured
mouse melanocyte line, melan-a, in 8 days (p < 0.01). piperine
(1-piperoylpiperidine),
the main alkaloid from piper nigrum fruit, also significantly
stimulated melan-a cell growth.
both piper nigrum extract and piperine induced morphological
alterations in melan-a cells,
with more and longer dendrites observed. the augmentation of
growth by piperine was
effectively inhibited by RO-31-8220, a selective protein kinase C
(PKC) inhibitor,
suggesting that PKC signalling is involved in its activity. this
is the first full report on such
an activity of black pepper and piperine.
[19]
phytochemistry
1997 45(8): 1617-1619
"an amide from fruits of piper nigrum"
bina s siddiqui, sabira begum, tahsin gulzar, farhat noor, fatima
noor
abstract: a new amide, N-isobutyl amide
of octadeca-trans-2-cis-4-dienoic acid
has been isolated from the dried and crushed fruits of piper
nigrum, along with the
known alkaloid, piperine. complete assignments of the protons and
carbons of the
new amide and piperine have been made based on 2D NMR studies.
[21]
patent US4820517, 1989-04-11
"process for obtaining a pepper extract with
insecticidal activity"
HANS PFEIFFER, MANFRED BIERMANN, PETER SCHROEDER,
GERD GOEBEL, ANNEMARIE MUELLER; HENKEL KGAA
abstract: PCT No. PCT/EP85/00333 Sec. 371
Date Jul. 24, 1986 Sec. 102(e) Date
Jul. 24, 1986 PCT Filed Jul. 6, 1985 PCT Pub. No. WO86/01981 PCT
Pub. Date Apr.
10, 1986.An insecticidally active fraction is obtained from black
pepper by a process
comprising the following steps: (a) extraction of black pepper in
ground form with CO2
at 30 to 70°C and 150 to 500 bar; (b) removal of sharp tasting
fractions therein in a first
expansion step at 25 to 35°C and 70 to 150 bar; (c) removal of
an oily fraction containing
the insecticidally active components as well as most of the
essential oils in a second
expansion step at 15 to 30°C and 40 to 70 bar; (d) removal of
essential oils by steam
distillation, and if desired; (e) hydrogenation of the
insecticidally active components.
[23]
patent EP0023680, 1981-02-11
"process for the production of spice extracts"
NORBERT BEHR, HENK VAN DER MEI, WOLFGANG SIRTL, DR HARALD
SCHNEGELBERGER, DR OTHMAR VON ETTINGSHAUSEN; HENKEL KGAA
abstract: 1. aprocess
for the production of spice extracts by extraction with a
physiologically acceptable gas as a solvent in two stages,
characterised in that the
ethereal oils acting as odour component are removed from the
spices in the first stage
using a liquid gas of which the temperature is in the subcritical
range and the pressure
in the supercritical range whilst the flavour components are
removed in the second
stage using the same gas of which the temperature and pressure
are both in the
supercritical range, the extracts are separated off from the
solutions obtained by
changing the pressure and/or temperature and optionally mixed
with one another.
[26]
(1)
piperine from black pepper (2) hydrolysis of piperine to piperic
acid
http://www.rhodium.ws/chemistry/piperine.txt
[27]
"piperine
to piperonal procedure", the cook
http://www.rhodium.ws/chemistry/piperine.txt
[31]
ind
eng chem res 2002 41(12): 2966-2976
"extraction of piperine from piper nigrum (black
pepper)
by hydrotropic solubilization"
girija raman, vilas g gaikar
abstract: hydrotropes, such as sodium
alkyl benzene sulfonates and sodium butyl
monoglycol sulfate, were used for the selective extraction of
piperine by cell
permeabilization of piper nigrum fruits. penetration of the
hydrotrope molecules into
the cellular structures and subsequent cell permeabilization were
hypothesized to
explain the enhanced extraction rates of aqueous hydrotrope
solutions. hydrotrope
molecules, after adsorption on a cell wall, cause disorder in its
structure and in the
bilayered cell membrane to facilitate the rapid extraction of
piperine. the hydrotrope
solution showed selective and rapid extraction of piperine from
black pepper.
the recovered piperine was ~90% pure and substantially free from
oleoresins. the
type and nature of the hydrotrope, the hydrotrope concentration,
the temperature,
and the particle size all had significant effects on the
extraction process.
[32]
patent CN1294127, 2001-05-09
"process for preparing piperine"
SHI ZAIFENG, SUN ZHENFAN, LIN XIAOMING; LUTIANYUAN HI TECH DEV CO
LTD
abstract: A process for preparing
piperine includes immersing extraction of pepper is
mixed organic solvent, distilling to separate out organic solvent
to obtain liquid mixture
of pepper oil resin and piperine, cooling to separate out the
pepper oil resin, and further
purifying. Its advantages are simple process, high extraction
rate and separation effect,
and high purity.
[33]
patent US4985265, 1991-01-15
"process for extraction of spices"
DUBOC SORAYA, RENON HENRI, LAUGIER SERGE, MIZANDJIAN JEAN-LUC,
PEAN JEAN-LOUIS; L AIR LIQUIDE SOCIETY ANONYME
abstract: a process for extracting spices
in liquid carbon dioxide in the presence
of an alimentarily acceptable cosolvent which does not degrade
the flavors of the
spices. the process is particularly applicable to the extraction
of piperine and
aromatic compounds of pepper.
[34]
patent CN1298936, 2001-06-13
"process for preparing health-care peperic acid wine";
li yueting
abstract: the present invention relates
to a preparation technology of piperic-acid
health-care wine which uses pepper or piper longum as raw
material, and it is
extracted and mixed with wine to obtain the product which not
only possesses wine
flavour, but also possesses the medicinal health-care active of
pepper or piper longum.
it can prevent the rise of blood serum cholesterol and formation
of choleithiasis.
[35]
boll chim farmaceutico 1998 137(8): 319-320
"long chain esters and alkamides from piper longum"
b das, a kashinatham, p madhusudhan
abstract: from the fruits of piper longum
two long chain esters,
tridecyldihydro-p-coumarate and eicosanyl-(E)-p-coumarate and
three alkamides,
piperine, piperlongumine and pellitorine have been isolated. the
first ester is a
new compound and the second ester is reported for the first time
from this plant.