Erowid References Database
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Segal M.
“5-HT antagonists in rat hippocampus”.
Brain Res.. 1976;103:161-166.
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Abstract
The monoamine serotonin (5-hydroxytryptamine, 5-HT) is presumed to be a neurotransmitter in teh central nervous system. Despite the existence of a large number of antagonists of serotonin, most of them have not thus far gained acceptance as satisfactory antagonists of serotonin in the brain. The present study attempted to evaluate serotonin antagonism on the ypramidal cells of the hippocampus (HPC) which receive serotonin containing afferents from the raphe nuclei. Ninety four adult (150-250 g) male Sprague-Dawley rats (obtained from Zivic-Miller Laboratories, Allison Park, Pa.) were used. The surgical and electrophysiological methods employed are presented elsewhere. The following drugs were used for iontophoresis: acetylcholine chloride (ACh, Merk, 2.5 M); bicuculline (Pierce Chem., 5 mg/ml, pH=4.0); bromolysergic acid diethylamide bitartrate (BOL-148, 10 mg/ml, DHEW, NIMH); lysergic acid diethylamide bitartrate (LSD, 1 mg/ml in 0.165 M NaCl, K and K); methiothepin maleate (Hoffman-La Roche, 10 mg/ml); cyproheptadine HCl (0.01 M, Merck); minanserin (0.01 M, Squibb); serotonin creatinine sulphate (5-HT, Aldrich, 0.5 M, pH=5); cinnanserin (0.01 M, Organon, Oss, Holland). All drugs were dissolved in distilled water or in saline and had a pH range of 4.0-8.0. In some cases, whern recording was made from a very slow-firing cell (less than 1-2 spikes/sec) continuous leakage of ACh was used in order to maintain a stable and faster firing rate (5-10 spikes/sec). This procedure enhanced detection of inhibitory responses, and protected against pre-selection of spontaneously rapid-firing cells for study. Care was taken to exclude the possibility that some drug effects could be due to interactions with ACh by recording from spontaneousl active cells and by comparing drug effects on some cells in the presence and absence of ACh leakage effects. When drug effects on the
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